Primers for phylogeny reconstruction in Bignonieae (Bignoniaceae) using herbarium samples1

UNLABELLED PREMISE OF THE STUDY New primers were developed for Bignonieae to enable phylogenetic studies within this clade using herbarium samples. • METHODS AND RESULTS Internal primers were designed based on available sequences of the plastid ndhF gene and the rpl32-trnL intergenic spacer region, and the nuclear gene PepC. The resulting primers were used to amplify DNA extracted from herbarium materials. High-quality data were obtained from herbarium samples up to 53 yr old. • CONCLUSIONS The standardized methodology allows the inclusion of herbarium materials as alternative sources of DNA for phylogenetic studies in Bignonieae.